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CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice

BACKGROUND: Maladaptation of the HPA (hypothalamic–pituitary–adrenal) axis plays an important role in depression-like behaviour, but the specific molecular mechanisms are unknown. Here, we determined the roles of CRHR1 (corticotrophin releasing hormone receptor 1) and nectin3 in LPS (lipopolysacchar...

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Autores principales: Sun, Jie, Qiu, Lili, Zhang, Hui, Zhou, Zhiqiang, Ju, Lingsha, Yang, Jiaojiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830857/
https://www.ncbi.nlm.nih.gov/pubmed/36624454
http://dx.doi.org/10.1186/s12888-023-04519-z
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author Sun, Jie
Qiu, Lili
Zhang, Hui
Zhou, Zhiqiang
Ju, Lingsha
Yang, Jiaojiao
author_facet Sun, Jie
Qiu, Lili
Zhang, Hui
Zhou, Zhiqiang
Ju, Lingsha
Yang, Jiaojiao
author_sort Sun, Jie
collection PubMed
description BACKGROUND: Maladaptation of the HPA (hypothalamic–pituitary–adrenal) axis plays an important role in depression-like behaviour, but the specific molecular mechanisms are unknown. Here, we determined the roles of CRHR1 (corticotrophin releasing hormone receptor 1) and nectin3 in LPS (lipopolysaccharide)-induced depression-like behaviour in mice. METHODS: C57BL/6 male mice were intraperitoneally injected with LPS (0.83 g/kg), and the open field, novelty-suppressed feeding, forced swimming, and tail suspension tests were performed after intraperitoneal injections of saline or antalarmin (20 mg/kg). The hippocampal mRNA levels of CRHR1 and nectin3 were determined by quantitative reverse transcription-PCR. The hippocampal protein levels of CRHR1, nectin3, and calbindin were measured by western blotting. The CORT (corticosterone) levels in the blood were measured by ELISA kits. RESULTS: Antalarmin alleviated LPS-induced depression-like behaviour in male mice. Furthermore, antalarmin significantly inhibited changes in CRHR1, nectin3 and calbindin levels in the hippocampus and reduced the increase in CORT levels in LPS-treated mice. CONCLUSION: CRHR1antagonist showed antidepressant effects in LPS-induced depressive mice, and CRHR1/nectin3 signalling may play a crucial role in this process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12888-023-04519-z.
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spelling pubmed-98308572023-01-11 CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice Sun, Jie Qiu, Lili Zhang, Hui Zhou, Zhiqiang Ju, Lingsha Yang, Jiaojiao BMC Psychiatry Research BACKGROUND: Maladaptation of the HPA (hypothalamic–pituitary–adrenal) axis plays an important role in depression-like behaviour, but the specific molecular mechanisms are unknown. Here, we determined the roles of CRHR1 (corticotrophin releasing hormone receptor 1) and nectin3 in LPS (lipopolysaccharide)-induced depression-like behaviour in mice. METHODS: C57BL/6 male mice were intraperitoneally injected with LPS (0.83 g/kg), and the open field, novelty-suppressed feeding, forced swimming, and tail suspension tests were performed after intraperitoneal injections of saline or antalarmin (20 mg/kg). The hippocampal mRNA levels of CRHR1 and nectin3 were determined by quantitative reverse transcription-PCR. The hippocampal protein levels of CRHR1, nectin3, and calbindin were measured by western blotting. The CORT (corticosterone) levels in the blood were measured by ELISA kits. RESULTS: Antalarmin alleviated LPS-induced depression-like behaviour in male mice. Furthermore, antalarmin significantly inhibited changes in CRHR1, nectin3 and calbindin levels in the hippocampus and reduced the increase in CORT levels in LPS-treated mice. CONCLUSION: CRHR1antagonist showed antidepressant effects in LPS-induced depressive mice, and CRHR1/nectin3 signalling may play a crucial role in this process. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12888-023-04519-z. BioMed Central 2023-01-09 /pmc/articles/PMC9830857/ /pubmed/36624454 http://dx.doi.org/10.1186/s12888-023-04519-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sun, Jie
Qiu, Lili
Zhang, Hui
Zhou, Zhiqiang
Ju, Lingsha
Yang, Jiaojiao
CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title_full CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title_fullStr CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title_full_unstemmed CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title_short CRHR1 antagonist alleviates LPS-induced depression-like behaviour in mice
title_sort crhr1 antagonist alleviates lps-induced depression-like behaviour in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830857/
https://www.ncbi.nlm.nih.gov/pubmed/36624454
http://dx.doi.org/10.1186/s12888-023-04519-z
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