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Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing

RNA polyadenylation plays a central role in RNA maturation, fate, and stability. In response to developmental cues, polyA tail lengths can vary, affecting the translation efficiency and stability of mRNAs. Here we develop Nanopore 3′ end-capture sequencing (Nano3P-seq), a method that relies on nanop...

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Detalles Bibliográficos
Autores principales: Begik, Oguzhan, Diensthuber, Gregor, Liu, Huanle, Delgado-Tejedor, Anna, Kontur, Cassandra, Niazi, Adnan Muhammad, Valen, Eivind, Giraldez, Antonio J., Beaudoin, Jean-Denis, Mattick, John S., Novoa, Eva Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9834059/
https://www.ncbi.nlm.nih.gov/pubmed/36536091
http://dx.doi.org/10.1038/s41592-022-01714-w