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A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain prote...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836081/ https://www.ncbi.nlm.nih.gov/pubmed/34285130 http://dx.doi.org/10.1126/scitranslmed.abg1143 |
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| author | Wu, Yangtao Huang, Xiaofen Yuan, Lunzhi Wang, Shaojuan Zhang, Yali Xiong, Hualong Chen, Rirong Ma, Jian Qi, Ruoyao Nie, Meifeng Xu, Jingjing Zhang, Zhigang Chen, Liqiang Wei, Min Zhou, Ming Cai, Minping Shi, Yang Zhang, Liang Yu, Huan Hong, Junping Wang, Zikang Hong, Yunda Yue, Mingxi Li, Zonglin Chen, Dabing Zheng, Qingbing Li, Shaowei Chen, Yixin Cheng, Tong Zhang, Jun Zhang, Tianying Zhu, Huachen Zhao, Qinjian Yuan, Quan Guan, Yi Xia, Ningshao |
| author_facet | Wu, Yangtao Huang, Xiaofen Yuan, Lunzhi Wang, Shaojuan Zhang, Yali Xiong, Hualong Chen, Rirong Ma, Jian Qi, Ruoyao Nie, Meifeng Xu, Jingjing Zhang, Zhigang Chen, Liqiang Wei, Min Zhou, Ming Cai, Minping Shi, Yang Zhang, Liang Yu, Huan Hong, Junping Wang, Zikang Hong, Yunda Yue, Mingxi Li, Zonglin Chen, Dabing Zheng, Qingbing Li, Shaowei Chen, Yixin Cheng, Tong Zhang, Jun Zhang, Tianying Zhu, Huachen Zhao, Qinjian Yuan, Quan Guan, Yi Xia, Ningshao |
| author_sort | Wu, Yangtao |
| collection | PubMed |
| description | Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate–modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both T(H)1- and T(H)2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by the absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit–based SARS-CoV-2 vaccine candidate. |
| format | Online Article Text |
| id | pubmed-9836081 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | American Association for the Advancement of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-98360812023-01-13 A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters Wu, Yangtao Huang, Xiaofen Yuan, Lunzhi Wang, Shaojuan Zhang, Yali Xiong, Hualong Chen, Rirong Ma, Jian Qi, Ruoyao Nie, Meifeng Xu, Jingjing Zhang, Zhigang Chen, Liqiang Wei, Min Zhou, Ming Cai, Minping Shi, Yang Zhang, Liang Yu, Huan Hong, Junping Wang, Zikang Hong, Yunda Yue, Mingxi Li, Zonglin Chen, Dabing Zheng, Qingbing Li, Shaowei Chen, Yixin Cheng, Tong Zhang, Jun Zhang, Tianying Zhu, Huachen Zhao, Qinjian Yuan, Quan Guan, Yi Xia, Ningshao Sci Transl Med Research Articles Multiple safe and effective vaccines that elicit immune responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are necessary to respond to the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a protein subunit vaccine composed of spike ectodomain protein (StriFK) plus a nitrogen bisphosphonate–modified zinc-aluminum hybrid adjuvant (FH002C). StriFK-FH002C generated substantially higher neutralizing antibody titers in mice, hamsters, and cynomolgus monkeys than those observed in plasma isolated from COVID-19 convalescent individuals. StriFK-FH002C also induced both T(H)1- and T(H)2-polarized helper T cell responses in mice. In hamsters, StriFK-FH002C immunization protected animals against SARS-CoV-2 challenge, as shown by the absence of virus-induced weight loss, fewer symptoms of disease, and reduced lung pathology. Vaccination of hamsters with StriFK-FH002C also reduced within-cage virus transmission to unvaccinated, cohoused hamsters. In summary, StriFK-FH002C represents an effective, protein subunit–based SARS-CoV-2 vaccine candidate. American Association for the Advancement of Science 2021-08-11 2021-07-20 /pmc/articles/PMC9836081/ /pubmed/34285130 http://dx.doi.org/10.1126/scitranslmed.abg1143 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Research Articles Wu, Yangtao Huang, Xiaofen Yuan, Lunzhi Wang, Shaojuan Zhang, Yali Xiong, Hualong Chen, Rirong Ma, Jian Qi, Ruoyao Nie, Meifeng Xu, Jingjing Zhang, Zhigang Chen, Liqiang Wei, Min Zhou, Ming Cai, Minping Shi, Yang Zhang, Liang Yu, Huan Hong, Junping Wang, Zikang Hong, Yunda Yue, Mingxi Li, Zonglin Chen, Dabing Zheng, Qingbing Li, Shaowei Chen, Yixin Cheng, Tong Zhang, Jun Zhang, Tianying Zhu, Huachen Zhao, Qinjian Yuan, Quan Guan, Yi Xia, Ningshao A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title | A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title_full | A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title_fullStr | A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title_full_unstemmed | A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title_short | A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters |
| title_sort | recombinant spike protein subunit vaccine confers protective immunity against sars-cov-2 infection and transmission in hamsters |
| topic | Research Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836081/ https://www.ncbi.nlm.nih.gov/pubmed/34285130 http://dx.doi.org/10.1126/scitranslmed.abg1143 |
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