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An efficient and cost-effective method for directed mutagenesis at multiple dispersed sites—a case study with Omicron Spike DNA
Site-directed mutagenesis is an invaluable technique that enables the elucidation of the contribution of specific residues to protein structure and function. The simultaneous introduction of mutations at a large number of sites (>10), singly and in multiple combinations, is often necessary to ful...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838316/ https://www.ncbi.nlm.nih.gov/pubmed/36654942 http://dx.doi.org/10.1093/biomethods/bpac037 |