Cargando…

Human butyrylcholinesterase in Cohn fraction IV-4 purified in a single chromatography step on Hupresin

Protection from the toxicity of nerve agents is achieved by pretreatment with human butyrylcholinesterase (BChE). Current methods for purifying large quantities of BChE from frozen Cohn fraction IV-4 produce 99% pure enzyme, but the yield is low (21%). Our goal was to simplify the purification proce...

Descripción completa

Detalles Bibliográficos
Autores principales:	M. Schopfer, Lawrence, David, Emilie, Hinrichs, Steven H., Lockridge, Oksana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838835/ https://www.ncbi.nlm.nih.gov/pubmed/36638134 http://dx.doi.org/10.1371/journal.pone.0280380

Ejemplares similares

Purification of human butyrylcholinesterase from frozen Cohn fraction IV-4 by ion exchange and Hupresin affinity chromatography
por: Schopfer, Lawrence M., et al.
Publicado: (2019)

Hupresin Retains Binding Capacity for Butyrylcholinesterase and Acetylcholinesterase after Sanitation with Sodium Hydroxide
por: Onder, Seda, et al.
Publicado: (2017)

Tetramer organizing polyproline-rich peptides identified by mass spectrometry after release of the peptides from Hupresin-purified butyrylcholinesterase tetramers isolated from milk of domestic pig (Sus scrofa)
por: Saxena, Ashima, et al.
Publicado: (2018)

Use of Hupresin To Capture Red Blood Cell Acetylcholinesterase for Detection of Soman Exposure
por: Onder, Seda, et al.
Publicado: (2017)

Chlorpyrifos Oxon-Induced Isopeptide Bond Formation in Human Butyrylcholinesterase
por: Biberoglu, Kevser, et al.
Publicado: (2020)

The C5 Variant of the Butyrylcholinesterase Tetramer Includes a Noncovalently Bound 60 kDa Lamellipodin Fragment
por: Schopfer, Lawrence M., et al.
Publicado: (2017)

Delipidation of Plasma Has Minimal Effects on Human Butyrylcholinesterase
por: Onder, Seda, et al.
Publicado: (2018)

Zanvil Alexander Cohn 1926-1993
Publicado: (1994)

Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity
por: Schopfer, Lawrence M., et al.
Publicado: (2018)

Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine
por: Schopfer, Lawrence M., et al.
Publicado: (2020)

Naturally Occurring Epsilon Gamma Glutamyl Lysine Isopeptide Crosslinks in Human Neuroblastoma SH-SY5Y Cells
por: Lockridge, Oksana, et al.
Publicado: (2022)

Dichlorvos-induced formation of isopeptide crosslinks between proteins in SH-SY5Y cells
por: Schopfer, Lawrence M., et al.
Publicado: (2022)

Monoclonal Antibodies That Recognize Various Folding States of Pure Human Butyrylcholinesterase Can Immunopurify Butyrylcholinesterase from Human Plasma Stored at Elevated Temperatures
por: Peng, Hong, et al.
Publicado: (2016)

Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
por: Schopfer, Lawrence M., et al.
Publicado: (2021)

Organophosphorus Pesticides Promote Protein Cross-Linking
por: Schopfer, Lawrence M., et al.
Publicado: (2022)

DDT and Breast Cancer: Cohn et al. Respond
por: Cohn, Barbara A., et al.
Publicado: (2008)

Generalization of the Calogero-Cohn bound on the number of bound states
por: Chadan, K., et al.
Publicado: (1995)

A Conversation with Cohn on the Activation of CD4 T Cells
por: Bretscher, P. A.
Publicado: (2015)

Chlorpyrifos Oxon Activates Glutamate and Lysine for Protein Cross-linking
por: Muñoz-Torrero, Diego, et al.
Publicado: (2023)

Naturally Occurring Genetic Variants of Human Acetylcholinesterase and Butyrylcholinesterase and Their Potential Impact on the Risk of Toxicity from Cholinesterase Inhibitors
por: Lockridge, Oksana, et al.
Publicado: (2016)

The Third Stage of Labour: Criticism of Papers by Cohn, Champneys, and Berry Hart
por: Barbour, A. H. F.
Publicado: (1888)

Draft Genome Sequence of Micrococcus luteus (Schroeter) Cohn (ATCC 12698)
por: Putonti, Catherine, et al.
Publicado: (2017)

Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography
por: Wang, Hua-zhen, et al.
Publicado: (2015)

The Third Stage of Labour: A Criticism of Papers by Cohn, Champneys, and Berry Hart
por: Barbour, A. H. F.
Publicado: (1888)

“Go no Go” in plasma fractionation in the world's emerging economies: Still a question asked 70 years after the COHN process was developed!
por: Burnouf, Thierry, et al.
Publicado: (2014)

Butyrylcholinesterase genotype and enzyme activity in relation to Gulf War illness: preliminary evidence of gene-exposure interaction from a case–control study of 1991 Gulf War veterans
por: Steele, Lea, et al.
Publicado: (2015)

An introduction to the theory of algebraic surfaces: notes by James Cohn, Harvard University, 1957–58
por: Zariski, Oscar
Publicado: (1969)

Sodium Ion Effect on Separation Of Butyrylcholinesterase from Plasma by Ion-Exchange Chromatography
por: POSTOARCA, A.G., et al.
Publicado: (2015)

Mass Spectral Detection of Diethoxyphospho-Tyrosine Adducts on Proteins from HEK293 Cells Using Monoclonal Antibody depY for Enrichment
por: Onder, Seda, et al.
Publicado: (2018)

Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry
por: Onder, Seda, et al.
Publicado: (2021)

Characterization of a Novel BCHE “Silent” Allele: Point Mutation (p.Val204Asp) Causes Loss of Activity and Prolonged Apnea with Suxamethonium
por: Delacour, Herve, et al.
Publicado: (2014)

Evolution of Acetylcholinesterase and Butyrylcholinesterase in the Vertebrates: An Atypical Butyrylcholinesterase from the Medaka Oryzias latipes
por: Pezzementi, Leo, et al.
Publicado: (2011)

Five Tyrosines and Two Serines in Human Albumin Are Labeled by the Organophosphorus Agent FP-Biotin
por: Ding, Shi-Jian, et al.
Publicado: (2008)

Identification of the AntiListerial Constituents in Partially Purified Column Chromatography Fractions of Garcinia kola Seeds and Their Interactions with Standard Antibiotics
por: Penduka, D., et al.
Publicado: (2014)

Optimization of Cholinesterase-Based Catalytic Bioscavengers Against Organophosphorus Agents
por: Lushchekina, Sofya V., et al.
Publicado: (2018)

Correction: Evolution of Acetylcholinesterase and Butyrylcholinesterase in the Vertebrates: An Atypical Butyrylcholinesterase from the Medaka Oryzias latipes
por: Pezzementi, Leo, et al.
Publicado: (2011)

Rapid Mechanistic Evaluation and Parameter Estimation of Putative Inhibitors in a Single-Step Progress-Curve Analysis: The Case of Horse Butyrylcholinesterase
por: Stojan, Jure
Publicado: (2017)

Molecular forms of butyrylcholinesterase and obesity
por: Boberg, Dellyana R., et al.
Publicado: (2010)

Caffeine Inhibits Acetylcholinesterase, But Not Butyrylcholinesterase
por: Pohanka, Miroslav, et al.
Publicado: (2013)

Electrochemical MIP Sensor for Butyrylcholinesterase
por: Ozcelikay, Goksu, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_11soa2u5fsaois3o1nbi05nac1