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Human DT-diaphorase expression in Escherichia coli: optimization, purification and structural stability

Expression and purification of human DT-diaphorase, also referred to as NAD(P)H quinone oxidoreductase 1 (NQO1; EC. 1.6.99.2), which is a flavoprotein belongs to the family of oxidoreductases are optimized. The DT-diaphorase plays an important role in biosensor design for laboratory analysis and als...

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Detalles Bibliográficos
Autores principales: Rouh, Bita, Seyedalipour, Bagher, Hosseinkhani, Saman, Hosseinzadeh Colagar, Abasalt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9840801/
https://www.ncbi.nlm.nih.gov/pubmed/36686868
http://dx.doi.org/10.30466/vrf.2021.527373.3159