Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysates

Here, we provide a protocol using chemical pulldown combined with mass spectrometry (LC-MS/MS) to identify drug targets in Plasmodium falciparum. This approach works upon the principle that a resin-bound inhibitor selectively binds its molecular target(s) in cell-free lysates. We describe the prepar...

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Detalles Bibliográficos
Autores principales: Smith, Robert J., Milne, Rachel, Lopez, Victoriano Corpas, Wiedemar, Natalie, Dey, Gourav, Syed, Aisha J., Patterson, Stephen, Wyllie, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841287/
https://www.ncbi.nlm.nih.gov/pubmed/36609153
http://dx.doi.org/10.1016/j.xpro.2022.102002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841287/
https://www.ncbi.nlm.nih.gov/pubmed/36609153
http://dx.doi.org/10.1016/j.xpro.2022.102002

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