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Chemical pulldown combined with mass spectrometry to identify the molecular targets of antimalarials in cell-free lysates
Here, we provide a protocol using chemical pulldown combined with mass spectrometry (LC-MS/MS) to identify drug targets in Plasmodium falciparum. This approach works upon the principle that a resin-bound inhibitor selectively binds its molecular target(s) in cell-free lysates. We describe the prepar...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9841287/ https://www.ncbi.nlm.nih.gov/pubmed/36609153 http://dx.doi.org/10.1016/j.xpro.2022.102002 |