Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR

Here, a method using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER) for miRNAs quantification was established. The strategy has two steps: (1) ligation of two DNA probes specifically hybridize to target miRNA and (2) qPCR amplifying the ligated probe....

Descripción completa

Detalles Bibliográficos
Autores principales: Qin, Xinshu, Wang, Xingyu, Xu, Ke, Zhang, Yi, Tian, Hongye, Li, Yinglei, Qi, Bangran, Yang, Xingbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842969/
https://www.ncbi.nlm.nih.gov/pubmed/36700047
http://dx.doi.org/10.1016/j.omtn.2022.12.015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9842969/
https://www.ncbi.nlm.nih.gov/pubmed/36700047
http://dx.doi.org/10.1016/j.omtn.2022.12.015

Ejemplares similares