Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells

Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined. Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases usin...

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Detalles Bibliográficos
Autores principales: Calle, Beatriz, Gonzalez-Rodriguez, Edgar, Mahoney, Keira E., Cioce, Anna, Bineva-Todd, Ganka, Tastan, Omur Y., Roustan, Chloe, Flynn, Helen, Malaker, Stacy A., Schumann, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843269/
https://www.ncbi.nlm.nih.gov/pubmed/36633947
http://dx.doi.org/10.1016/j.xpro.2022.101974

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843269/
https://www.ncbi.nlm.nih.gov/pubmed/36633947
http://dx.doi.org/10.1016/j.xpro.2022.101974

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