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Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization

BACKGROUND: Tumor-associated macrophages (TAMs) affects the outcomes of non-small cell lung cancer (NSCLC). NSCLC cells released exosomes to suppress the antitumor activity of TAMs. MiR-146a is a critical regulator in TAM polarization. We hypothesized that NSCLC cells released exosomal miR-146a to r...

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Autores principales: Yan, Zhi, Wen, Jian-Xun, Cao, Xi-Shan, Zhao, Wen, Han, Yu-Ling, Wen, Xu-Hui, Yan, Li, Zhang, Man, Wang, Ya-Fei, Hai, Ling, Wang, Ying-Jun, Zheng, Wen-Qi, Hu, Zhi-De
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843328/
https://www.ncbi.nlm.nih.gov/pubmed/36660623
http://dx.doi.org/10.21037/atm-22-5565
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author Yan, Zhi
Wen, Jian-Xun
Cao, Xi-Shan
Zhao, Wen
Han, Yu-Ling
Wen, Xu-Hui
Yan, Li
Zhang, Man
Wang, Ya-Fei
Hai, Ling
Wang, Ying-Jun
Zheng, Wen-Qi
Hu, Zhi-De
author_facet Yan, Zhi
Wen, Jian-Xun
Cao, Xi-Shan
Zhao, Wen
Han, Yu-Ling
Wen, Xu-Hui
Yan, Li
Zhang, Man
Wang, Ya-Fei
Hai, Ling
Wang, Ying-Jun
Zheng, Wen-Qi
Hu, Zhi-De
author_sort Yan, Zhi
collection PubMed
description BACKGROUND: Tumor-associated macrophages (TAMs) affects the outcomes of non-small cell lung cancer (NSCLC). NSCLC cells released exosomes to suppress the antitumor activity of TAMs. MiR-146a is a critical regulator in TAM polarization. We hypothesized that NSCLC cells released exosomal miR-146a to regulate TAM polarization and thus affected its antitumor activity. METHODS: We used H1299 cells-derived exosomes to stimulate THP-1 cells that was pretreated with phorbol 12-myristate 13-acetate (M0 macrophage). Flow cytometry and reverse transcription-quantitative polymerase chain reaction (PCR) were used to determine the polarization of macrophages. The conditioned medium of exosome-treated M0 cells was used to culture H1299 cells, and the Cell Counting Kit-8, Ki67, transwell and scratch wound assays were used to determine the biological behavior of H1299 cells. To investigate whether exosomal miR-146a regulates TAM macrophages through targeting tumor necrosis factor receptor-associated factor 6 (TRAF-6) and interleukin-1 receptor-associated kinase 1 (IRAK-1), we used small interfering RNA to knockdown the expressions of them. RESULTS: Upregulation of miR-146a inhibited M1 polarization and thus impaired the antitumor activity of TAMs. Exosomes released by H1299 cells can be taken by M0 macrophage, and they upregulated the expression of miR-146a in M0 macrophage. The exosome suppresses M1 polarization by exosomal miR-146a. TRAF-6 and IRAK-1 mediated the inhibitive effects of exosomal miR-146a on M1 polarization. CONCLUSIONS: NSCLC cells released exosomal miR-146a to inhibit the expressions of TRAF-6 and IRAK-1 in TAMs, resulting in the impaired antitumor activity of TAMs. NSCLC cell-derived exosomal miR-146a represents a novel therapeutic target for NSCLC treatment.
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spelling pubmed-98433282023-01-18 Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization Yan, Zhi Wen, Jian-Xun Cao, Xi-Shan Zhao, Wen Han, Yu-Ling Wen, Xu-Hui Yan, Li Zhang, Man Wang, Ya-Fei Hai, Ling Wang, Ying-Jun Zheng, Wen-Qi Hu, Zhi-De Ann Transl Med Original Article BACKGROUND: Tumor-associated macrophages (TAMs) affects the outcomes of non-small cell lung cancer (NSCLC). NSCLC cells released exosomes to suppress the antitumor activity of TAMs. MiR-146a is a critical regulator in TAM polarization. We hypothesized that NSCLC cells released exosomal miR-146a to regulate TAM polarization and thus affected its antitumor activity. METHODS: We used H1299 cells-derived exosomes to stimulate THP-1 cells that was pretreated with phorbol 12-myristate 13-acetate (M0 macrophage). Flow cytometry and reverse transcription-quantitative polymerase chain reaction (PCR) were used to determine the polarization of macrophages. The conditioned medium of exosome-treated M0 cells was used to culture H1299 cells, and the Cell Counting Kit-8, Ki67, transwell and scratch wound assays were used to determine the biological behavior of H1299 cells. To investigate whether exosomal miR-146a regulates TAM macrophages through targeting tumor necrosis factor receptor-associated factor 6 (TRAF-6) and interleukin-1 receptor-associated kinase 1 (IRAK-1), we used small interfering RNA to knockdown the expressions of them. RESULTS: Upregulation of miR-146a inhibited M1 polarization and thus impaired the antitumor activity of TAMs. Exosomes released by H1299 cells can be taken by M0 macrophage, and they upregulated the expression of miR-146a in M0 macrophage. The exosome suppresses M1 polarization by exosomal miR-146a. TRAF-6 and IRAK-1 mediated the inhibitive effects of exosomal miR-146a on M1 polarization. CONCLUSIONS: NSCLC cells released exosomal miR-146a to inhibit the expressions of TRAF-6 and IRAK-1 in TAMs, resulting in the impaired antitumor activity of TAMs. NSCLC cell-derived exosomal miR-146a represents a novel therapeutic target for NSCLC treatment. AME Publishing Company 2022-12 /pmc/articles/PMC9843328/ /pubmed/36660623 http://dx.doi.org/10.21037/atm-22-5565 Text en 2022 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Yan, Zhi
Wen, Jian-Xun
Cao, Xi-Shan
Zhao, Wen
Han, Yu-Ling
Wen, Xu-Hui
Yan, Li
Zhang, Man
Wang, Ya-Fei
Hai, Ling
Wang, Ying-Jun
Zheng, Wen-Qi
Hu, Zhi-De
Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title_full Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title_fullStr Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title_full_unstemmed Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title_short Tumor cell-derived exosomal microRNA-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting M1 macrophage polarization
title_sort tumor cell-derived exosomal microrna-146a promotes non-small cell lung cancer cell invasion and proliferation by inhibiting m1 macrophage polarization
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843328/
https://www.ncbi.nlm.nih.gov/pubmed/36660623
http://dx.doi.org/10.21037/atm-22-5565
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