Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses

Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escheric...

Descripción completa

Detalles Bibliográficos
Autores principales: Vinchhi, Rhea, Jena, Chinmaya, Matange, Nishad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843481/
https://www.ncbi.nlm.nih.gov/pubmed/36625217
http://dx.doi.org/10.1016/j.xpro.2022.102005
_version_ 1784870415662841856
author Vinchhi, Rhea
Jena, Chinmaya
Matange, Nishad
author_facet Vinchhi, Rhea
Jena, Chinmaya
Matange, Nishad
author_sort Vinchhi, Rhea
collection PubMed
description Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escherichia coli that can be easily adapted to answer questions related to evolution and genetics of AMR in diverse bacteria. Key issues to consider when designing ALE experiments as well as some downstream mutation mapping analyses are described. For complete details on the use and execution of this protocol, please refer to Patel and Matange (2021)(1) and Matange et al. (2019).(2)
format Online
Article
Text
id pubmed-9843481
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-98434812023-01-18 Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses Vinchhi, Rhea Jena, Chinmaya Matange, Nishad STAR Protoc Protocol Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escherichia coli that can be easily adapted to answer questions related to evolution and genetics of AMR in diverse bacteria. Key issues to consider when designing ALE experiments as well as some downstream mutation mapping analyses are described. For complete details on the use and execution of this protocol, please refer to Patel and Matange (2021)(1) and Matange et al. (2019).(2) Elsevier 2023-01-09 /pmc/articles/PMC9843481/ /pubmed/36625217 http://dx.doi.org/10.1016/j.xpro.2022.102005 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Vinchhi, Rhea
Jena, Chinmaya
Matange, Nishad
Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title_full Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title_fullStr Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title_full_unstemmed Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title_short Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
title_sort adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843481/
https://www.ncbi.nlm.nih.gov/pubmed/36625217
http://dx.doi.org/10.1016/j.xpro.2022.102005
work_keys_str_mv AT vinchhirhea adaptivelaboratoryevolutionofantimicrobialresistanceinbacteriaforgeneticandphenotypicanalyses
AT jenachinmaya adaptivelaboratoryevolutionofantimicrobialresistanceinbacteriaforgeneticandphenotypicanalyses
AT matangenishad adaptivelaboratoryevolutionofantimicrobialresistanceinbacteriaforgeneticandphenotypicanalyses

Ejemplares similares