Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme

BACKGROUND: CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness. However, it remains unclear whether the efficiency of this technology can be further improved and app...

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Detalles Bibliográficos
Autores principales: Zhan, Yonghao, Cao, Congcong, Li, Aolin, Mei, Hongbing, Liu, Yuchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843992/
https://www.ncbi.nlm.nih.gov/pubmed/36650600
http://dx.doi.org/10.1186/s13059-023-02852-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9843992/
https://www.ncbi.nlm.nih.gov/pubmed/36650600
http://dx.doi.org/10.1186/s13059-023-02852-w

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