Optimized protocol for live-cell analysis of kinetochore fiber maturation in Indian muntjac cells

Here, we take advantage of the low chromosome number (2N=6) and distinctively large kinetochores of female Indian muntjac cells to investigate the molecular mechanism underlying k-fiber maturation. We describe steps for monitoring kinetochore-microtubule dynamics over time. Specifically, we detail t...

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Detalles Bibliográficos
Autores principales: Almeida, Ana Coelho, Soares-de-Oliveira, Joana, Maiato, Helder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846000/
https://www.ncbi.nlm.nih.gov/pubmed/36640366
http://dx.doi.org/10.1016/j.xpro.2022.102011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846000/
https://www.ncbi.nlm.nih.gov/pubmed/36640366
http://dx.doi.org/10.1016/j.xpro.2022.102011

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