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Optimized protocol for live-cell analysis of kinetochore fiber maturation in Indian muntjac cells

Here, we take advantage of the low chromosome number (2N=6) and distinctively large kinetochores of female Indian muntjac cells to investigate the molecular mechanism underlying k-fiber maturation. We describe steps for monitoring kinetochore-microtubule dynamics over time. Specifically, we detail t...

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Detalles Bibliográficos
Autores principales:	Almeida, Ana Coelho, Soares-de-Oliveira, Joana, Maiato, Helder
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846000/ https://www.ncbi.nlm.nih.gov/pubmed/36640366 http://dx.doi.org/10.1016/j.xpro.2022.102011

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