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Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the g...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846123/ https://www.ncbi.nlm.nih.gov/pubmed/36638019 http://dx.doi.org/10.1016/j.xpro.2022.102022 |