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Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis

The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the g...

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Detalles Bibliográficos
Autores principales: Richon, Sophie, Zajac, Olivier, Perez Gonzalez, Carlos, Matic Vignjevic, Danijela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846123/
https://www.ncbi.nlm.nih.gov/pubmed/36638019
http://dx.doi.org/10.1016/j.xpro.2022.102022