Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis

The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the g...

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Autores principales: Richon, Sophie, Zajac, Olivier, Perez Gonzalez, Carlos, Matic Vignjevic, Danijela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846123/
https://www.ncbi.nlm.nih.gov/pubmed/36638019
http://dx.doi.org/10.1016/j.xpro.2022.102022
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author Richon, Sophie
Zajac, Olivier
Perez Gonzalez, Carlos
Matic Vignjevic, Danijela
author_facet Richon, Sophie
Zajac, Olivier
Perez Gonzalez, Carlos
Matic Vignjevic, Danijela
author_sort Richon, Sophie
collection PubMed
description The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the growth of the primary tumor in real time using colonoscopy or in vivo imaging system, as well as monitoring metastasis development. Finally, we describe tissue collection and sample preparation for subsequent immunohistochemistry analysis.
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spelling pubmed-98461232023-01-19 Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis Richon, Sophie Zajac, Olivier Perez Gonzalez, Carlos Matic Vignjevic, Danijela STAR Protoc Protocol The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitoring the growth of the primary tumor in real time using colonoscopy or in vivo imaging system, as well as monitoring metastasis development. Finally, we describe tissue collection and sample preparation for subsequent immunohistochemistry analysis. Elsevier 2023-01-12 /pmc/articles/PMC9846123/ /pubmed/36638019 http://dx.doi.org/10.1016/j.xpro.2022.102022 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Richon, Sophie
Zajac, Olivier
Perez Gonzalez, Carlos
Matic Vignjevic, Danijela
Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title_full Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title_fullStr Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title_full_unstemmed Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title_short Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
title_sort optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9846123/
https://www.ncbi.nlm.nih.gov/pubmed/36638019
http://dx.doi.org/10.1016/j.xpro.2022.102022
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