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Genetic code expansion to enable site‐specific bioorthogonal labeling of functional G protein‐coupled receptors in live cells

For use in site‐specific bioorthogonal labeling of expressed G protein‐coupled receptors (GPCRs) in live cells, we developed a luciferase‐based reporter assay. The assay was used to compare amber codon suppression efficiency, receptor functionality, and efficiency of different bioorthogonal labeling...

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Detalles Bibliográficos
Autores principales: Mattheisen, Jordan M., Wollowitz, Jaina S., Huber, Thomas, Sakmar, Thomas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847076/
https://www.ncbi.nlm.nih.gov/pubmed/36540928
http://dx.doi.org/10.1002/pro.4550