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Genetic code expansion to enable site‐specific bioorthogonal labeling of functional G protein‐coupled receptors in live cells
For use in site‐specific bioorthogonal labeling of expressed G protein‐coupled receptors (GPCRs) in live cells, we developed a luciferase‐based reporter assay. The assay was used to compare amber codon suppression efficiency, receptor functionality, and efficiency of different bioorthogonal labeling...
Autores principales: | Mattheisen, Jordan M., Wollowitz, Jaina S., Huber, Thomas, Sakmar, Thomas P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847076/ https://www.ncbi.nlm.nih.gov/pubmed/36540928 http://dx.doi.org/10.1002/pro.4550 |
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