Cargando…

A modified high-resolution melting-based assay (HRM) to identify the SARS-CoV-2 N501Y variant

High-resolution melting (HRM) analysis is a PCR–based method that can be used as a screening assay to identify SARS-CoV-2 variants. However, conventional HRM assays hardly detect slight melting temperature differences at the A–T to T–A transversion. As the N501Y substitution results from A–T to T–A...

Descripción completa

Detalles Bibliográficos
Autores principales:	Aoki, Akira, Adachi, Hirokazu, Mori, Yoko, Ito, Miyabi, Sato, Katsuhiko, Kinoshita, Masayoshi, Kuriki, Masahiro, Okuda, Kenji, Sakakibara, Toru, Okamoto, Yoshinori, Jinno, Hideto
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Published by Elsevier B.V. 2023
Materias:	Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847321/ https://www.ncbi.nlm.nih.gov/pubmed/36681163 http://dx.doi.org/10.1016/j.jviromet.2023.114678

Ejemplares similares

Rapid Identification of SARS-CoV-2 Omicron BA.5 Spike Mutation F486V in Clinical Specimens Using a High-Resolution Melting-Based Assay
por: Aoki, Akira, et al.
Publicado: (2022)

Discrimination of SARS-CoV-2 Omicron Sublineages BA.1 and BA.2 Using a High-Resolution Melting-Based Assay: a Pilot Study
por: Aoki, Akira, et al.
Publicado: (2022)

Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis
por: Aoki, Akira, et al.
Publicado: (2021)

Assessment for Melting Temperature Measurement of Nucleic Acid by HRM
por: Wang, Jing, et al.
Publicado: (2016)

A Rapid Screening Assay for Clarithromycin-Resistant Mycobacterium avium Complex Using Melting Curve Analysis with Nonfluorescent Labeled Probes
por: Aoki, Akira, et al.
Publicado: (2023)

Age estimation using methylation-sensitive high-resolution melting (MS-HRM) in both healthy felines and those with chronic kidney disease
por: Qi, Huiyuan, et al.
Publicado: (2021)

Multi-locus identification of Psilocybe cubensis by high-resolution melting (HRM)
por: Zhang, Xiaochun, et al.
Publicado: (2021)

Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach
por: Santos, Guilherme Brzoskowski, et al.
Publicado: (2013)

Methylation levels assessment with Methylation-Sensitive High-Resolution Melting (MS-HRM)
por: Samsø Mathiasen, Sally, et al.
Publicado: (2022)

Detection of Somatic Mutations by High-Resolution DNA Melting (HRM) Analysis in Multiple Cancers
por: Gonzalez-Bosquet, Jesus, et al.
Publicado: (2011)

Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis
por: Álvarez-Sandoval, Brenda A., et al.
Publicado: (2014)

Distinguishing Anuran species by high‐resolution melting analysis of the COI barcode (COI‐HRM)
por: Everman, Steven, et al.
Publicado: (2019)

Global DNA Methylation in Cord Blood as a Biomarker for Prenatal Lead and Antimony Exposures
por: Okamoto, Yoshinori, et al.
Publicado: (2022)

Analysis of HIV Using a High Resolution Melting (HRM) Diversity Assay: Automation of HRM Data Analysis Enhances the Utility of the Assay for Analysis of HIV Incidence
por: Cousins, Matthew M., et al.
Publicado: (2012)

A Strategy to Setup Codominant Microsatellite Analysis for High-Resolution-Melting-Curve-Analysis (HRM)
por: Mader, Eduard, et al.
Publicado: (2008)

Rapid Detection and Identification of Human Hookworm Infections through High Resolution Melting (HRM) Analysis
por: Ngui, Romano, et al.
Publicado: (2012)

Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species
por: Song, Ming, et al.
Publicado: (2016)

High Resolution Melting (HRM) for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies
por: Słomka, Marcin, et al.
Publicado: (2017)

A Forensic Detection Method for Hallucinogenic Mushrooms via High-Resolution Melting (HRM) Analysis
por: Zhang, Xiaochun, et al.
Publicado: (2021)

Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification
por: Kriangwanich, Wannapimol, et al.
Publicado: (2021)

High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
por: Lochlainn, Seosamh Ó, et al.
Publicado: (2011)

Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis
por: Sakaridis, Ioannis, et al.
Publicado: (2014)

Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
por: Lamien-Meda, Aline, et al.
Publicado: (2019)

High-resolution melting (HRM) curve analysis as a potential tool for the identification of earthworm species and haplotypes
por: Vaupel, Anna, et al.
Publicado: (2022)

Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus
por: Narimisa, Negar, et al.
Publicado: (2022)

High-Resolution Melting (HRM) Analysis for Rapid Molecular Identification of Sparidae Species in the Greek Fish Market
por: Chatzoglou, Evanthia, et al.
Publicado: (2023)

Less Carcinogenic Chlorinated Estrogens Applicable to Hormone Replacement Therapy
por: Okamoto, Yoshinori, et al.
Publicado: (2021)

Strategic prospects for HRM
por: Tyson, Shaun
Publicado: (1995)

An efficient method for developing SNP markers based on EST data combined with high resolution melting (HRM) analysis
por: Ujino-Ihara, Tokuko, et al.
Publicado: (2010)

Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis
por: Hanson, Erin K., et al.
Publicado: (2014)

Rapid and specific detection of porcine parvovirus using real-time PCR and High Resolution Melting (HRM) analysis
por: Yu, Hai-Qiong, et al.
Publicado: (2015)

Development of a new High Resolution Melting (HRM) assay for identification and differentiation of Mycobacterium tuberculosis complex samples
por: Landolt, Patricia, et al.
Publicado: (2019)

Analysis of the reannealing- instead of melting-curve in the detection of JAK2 V617F mutation by HRM method
por: Moradabadi, Alireza, et al.
Publicado: (2019)

Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
por: Ashrafi, Roghaieh, et al.
Publicado: (2017)

One-Step Differential Detection of OXA-48-Like Variants Using High-Resolution Melting (HRM) Analysis
por: Lau, Min Yi, et al.
Publicado: (2020)

High-Resolution Melting (HRM) Curve Assay for the Identification of Eight Fusarium Species Causing Ear Rot in Maize
por: Schiwek, Simon, et al.
Publicado: (2020)

Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples
por: Pakbin, Babak, et al.
Publicado: (2022)

Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation
por: Wojdacz, Tomasz K., et al.
Publicado: (2007)

Identification of Six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay
por: Hernández, Carolina, et al.
Publicado: (2014)

Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
por: Cłapa, Tomasz, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_s17b94lv6qeil2ju74t3pc9de3