Cargando…

Comparison of CRISPR-Cas9-mediated megabase-scale genome deletion methods in mouse embryonic stem cells

The genome contains large functional units ranging in size from hundreds of kilobases to megabases, such as gene clusters and topologically associating domains. To analyse these large functional units, the technique of deleting the entire functional unit is effective. However, deletion of such large...

Descripción completa

Detalles Bibliográficos
Autores principales:	Miyata, Masayuki, Yoshida, Junko, Takagishi, Itsuki, Horie, Kyoji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9847339/ https://www.ncbi.nlm.nih.gov/pubmed/36448318 http://dx.doi.org/10.1093/dnares/dsac045

Ejemplares similares

Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line
por: Essletzbichler, Patrick, et al.
Publicado: (2014)

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
por: Cullot, Grégoire, et al.
Publicado: (2019)

Generation of megabase-scale deletions, inversions and duplications involving the Contactin-6 gene in mice by CRISPR/Cas9 technology
por: Korablev, Alexei N., et al.
Publicado: (2017)

Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system
por: Kato, Tomoko, et al.
Publicado: (2017)

Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9
por: Cullot, G., et al.
Publicado: (2023)

Exploiting CRISPR/Cas9 to engineer precise segmental deletions in mouse embryonic stem cells
por: Elango, Rajula, et al.
Publicado: (2022)

CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells
por: Boutin, J., et al.
Publicado: (2021)

Time origin and structural analysis of the induced CRISPR/cas9 megabase-sized deletions and duplications involving the Cntn6 gene in mice
por: Pristyazhnyuk, Inna E., et al.
Publicado: (2019)

Engineering of targeted megabase-scale deletions in human induced pluripotent stem cells
por: Kotini, Andriana G., et al.
Publicado: (2020)

Engineering large-scale chromosomal deletions by CRISPR-Cas9
por: Eleveld, Thomas F, et al.
Publicado: (2021)

A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize
por: Han, Xuesong, et al.
Publicado: (2019)

Large-scale genomic deletions mediated by CRISPR/Cas9 system
por: Song, Yuning, et al.
Publicado: (2017)

CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA
por: Zhou, Jianting, et al.
Publicado: (2016)

CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells
por: Kimura, Yasuyoshi, et al.
Publicado: (2015)

Chromatin Is Frequently Unknotted at the Megabase Scale
por: Goundaroulis, Dimos, et al.
Publicado: (2020)

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9
por: Zhang, Luqing, et al.
Publicado: (2015)

CRISPR-mediated isolation of specific megabase segments of genomic DNA
por: Bennett-Baker, Pamela E., et al.
Publicado: (2017)

Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR
por: Liu, Yuhan, et al.
Publicado: (2022)

CRISPR-based targeted haplotype-resolved assembly of a megabase region
por: Li, Taotao, et al.
Publicado: (2023)

Generation of An Endogenous FGFR2–BICC1 Gene Fusion/58 Megabase Inversion Using Single-Plasmid CRISPR/Cas9 Editing in Biliary Cells
por: Reicher, Andreas, et al.
Publicado: (2020)

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
por: Amorim, João Pedro, et al.
Publicado: (2020)

Efficient Production of Biallelic RAG1 Knockout Mouse Embryonic Stem Cell Using CRISPR/Cas9
por: Mehravar, Maryam, et al.
Publicado: (2019)

On-Target CRISPR/Cas9 Activity Can Cause Undesigned Large Deletion in Mouse Zygotes
por: Korablev, Alexey, et al.
Publicado: (2020)

Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse
por: Pineault, Kyriel M., et al.
Publicado: (2019)

CRISPR/Cas9‐induced disruption of gene expression in mouse embryonic brain and single neural stem cells in vivo
por: Kalebic, Nereo, et al.
Publicado: (2016)

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome
por: Shin, Ha Youn, et al.
Publicado: (2017)

Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9
por: Jiang, Junfeng, et al.
Publicado: (2016)

CRISPR/Cas9-mediated genome editing in naïve human embryonic stem cells
por: Jacobs, Eva Z., et al.
Publicado: (2017)

Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells
por: Yin, Zixi, et al.
Publicado: (2017)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

CRISPR/Cas9-Mediated Deletion of Large Genomic Fragments in Soybean
por: Cai, Yupeng, et al.
Publicado: (2018)

CRISPR/Cas9 deletion of ORMDLs reveals complexity in sphingolipid metabolism
por: Green, Christopher D., et al.
Publicado: (2021)

Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma
por: Joehnk, Bastian, et al.
Publicado: (2023)

Postembryonic Establishment of Megabase-Scale Gene Silencing in Nucleolar Dominance
por: Pontes, Olga, et al.
Publicado: (2007)

Molecular Combing of Single DNA Molecules on the 10 Megabase Scale
por: Kaykov, Atanas, et al.
Publicado: (2016)

The megabase-scale crossover landscape is largely independent of sequence divergence
por: Lian, Qichao, et al.
Publicado: (2022)

Robust and efficient knock-in in embryonic stem cells and early-stage embryos of the common marmoset using the CRISPR-Cas9 system
por: Yoshimatsu, Sho, et al.
Publicado: (2019)

A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells
por: Paulis, Marianna, et al.
Publicado: (2015)

High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells
por: Carlson-Stevermer, Jared, et al.
Publicado: (2016)

CRISPR/Cas9-AAV Mediated Knock-in at NRL Locus in Human Embryonic Stem Cells
por: Ge, Xianglian, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_hi1cgrdo0bgvkdpidu2dn552cl