Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia

We describe an optimized protocol for application of expansion microscopy (ExM) on chick neural tube (NT) which enables different oriented nanoscale resolution imaging of the centrosomes/cilia. We explain embryo NT transversal sections and open-book preparations, immunohistochemistry for labeling, a...

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Detalles Bibliográficos
Autores principales: Wilmerding, Axelle, Espana-Bonilla, Paula, Giakoumakis, Nikolaos-Nikiforos, Saade, Murielle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850183/
https://www.ncbi.nlm.nih.gov/pubmed/36609151
http://dx.doi.org/10.1016/j.xpro.2022.101997

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850183/
https://www.ncbi.nlm.nih.gov/pubmed/36609151
http://dx.doi.org/10.1016/j.xpro.2022.101997

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