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Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96

Metabolic derangement is a key culprit in kidney pathophysiology. Organoids have emerged as a promising in vitro tool for kidney research. Here, we present a fine-tuned protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell (iPSC)-derived kidney organoids using Seahorse XF96...

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Detalles Bibliográficos
Autores principales: Miguel, Verónica, Reimer, Katharina Charlotte, Galyga, Anna Katharina, Jansen, Jitske, Möllmann, Julia, Meyer, Luisa, Schneider, Rebekka K., Kramann, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850189/
https://www.ncbi.nlm.nih.gov/pubmed/36607813
http://dx.doi.org/10.1016/j.xpro.2022.101999