Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96

Metabolic derangement is a key culprit in kidney pathophysiology. Organoids have emerged as a promising in vitro tool for kidney research. Here, we present a fine-tuned protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell (iPSC)-derived kidney organoids using Seahorse XF96...

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Autores principales: Miguel, Verónica, Reimer, Katharina Charlotte, Galyga, Anna Katharina, Jansen, Jitske, Möllmann, Julia, Meyer, Luisa, Schneider, Rebekka K., Kramann, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850189/
https://www.ncbi.nlm.nih.gov/pubmed/36607813
http://dx.doi.org/10.1016/j.xpro.2022.101999
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author Miguel, Verónica
Reimer, Katharina Charlotte
Galyga, Anna Katharina
Jansen, Jitske
Möllmann, Julia
Meyer, Luisa
Schneider, Rebekka K.
Kramann, Rafael
author_facet Miguel, Verónica
Reimer, Katharina Charlotte
Galyga, Anna Katharina
Jansen, Jitske
Möllmann, Julia
Meyer, Luisa
Schneider, Rebekka K.
Kramann, Rafael
author_sort Miguel, Verónica
collection PubMed
description Metabolic derangement is a key culprit in kidney pathophysiology. Organoids have emerged as a promising in vitro tool for kidney research. Here, we present a fine-tuned protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell (iPSC)-derived kidney organoids using Seahorse XF96. We describe the generation of self-organized three-dimensional kidney organoids, followed by preparation of organoids for Seahorse XF96 analysis. We then detail how to carry out stress tests to determine mitochondrial and glycolytic rates in single kidney organoids.
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spelling pubmed-98501892023-01-20 Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96 Miguel, Verónica Reimer, Katharina Charlotte Galyga, Anna Katharina Jansen, Jitske Möllmann, Julia Meyer, Luisa Schneider, Rebekka K. Kramann, Rafael STAR Protoc Protocol Metabolic derangement is a key culprit in kidney pathophysiology. Organoids have emerged as a promising in vitro tool for kidney research. Here, we present a fine-tuned protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell (iPSC)-derived kidney organoids using Seahorse XF96. We describe the generation of self-organized three-dimensional kidney organoids, followed by preparation of organoids for Seahorse XF96 analysis. We then detail how to carry out stress tests to determine mitochondrial and glycolytic rates in single kidney organoids. Elsevier 2023-01-05 /pmc/articles/PMC9850189/ /pubmed/36607813 http://dx.doi.org/10.1016/j.xpro.2022.101999 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Miguel, Verónica
Reimer, Katharina Charlotte
Galyga, Anna Katharina
Jansen, Jitske
Möllmann, Julia
Meyer, Luisa
Schneider, Rebekka K.
Kramann, Rafael
Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title_full Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title_fullStr Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title_full_unstemmed Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title_short Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
title_sort protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using seahorse xf96
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850189/
https://www.ncbi.nlm.nih.gov/pubmed/36607813
http://dx.doi.org/10.1016/j.xpro.2022.101999
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