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High‐yield recombinant bacterial expression of (13)C‐, (15)N‐labeled, serine‐16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol

Amelogenin constitutes ~90% of the enamel matrix in the secretory stage of amelogenesis, a still poorly understood process that results in the formation of the hardest and most mineralized tissue in vertebrates—enamel. Most biophysical research with amelogenin uses recombinant protein expressed in E...

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Detalles Bibliográficos
Autores principales: Buchko, Garry W., Zhou, Mowei, Vesely, Cat Hoang, Tao, Jinhui, Shaw, Wendy J., Mehl, Ryan A., Cooley, Richard B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850436/
https://www.ncbi.nlm.nih.gov/pubmed/36585836
http://dx.doi.org/10.1002/pro.4560