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High‐yield recombinant bacterial expression of (13)C‐, (15)N‐labeled, serine‐16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol
Amelogenin constitutes ~90% of the enamel matrix in the secretory stage of amelogenesis, a still poorly understood process that results in the formation of the hardest and most mineralized tissue in vertebrates—enamel. Most biophysical research with amelogenin uses recombinant protein expressed in E...
Autores principales: | Buchko, Garry W., Zhou, Mowei, Vesely, Cat Hoang, Tao, Jinhui, Shaw, Wendy J., Mehl, Ryan A., Cooley, Richard B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850436/ https://www.ncbi.nlm.nih.gov/pubmed/36585836 http://dx.doi.org/10.1002/pro.4560 |
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