Cargando…

Effects of Chemical Additives in Refolding Buffer on Recombinant Human BMP-2 Dimerization and the Bioactivity on SaOS-2 Osteoblasts

[Image: see text] Bone morphogenetic protein-2 (BMP-2) is a promising osteogenic agent in tissue engineering. BMP-2 is usually expressed in Escherichia coli owing to the high yield and low cost, but the protein is expressed as inclusion bodies. Thus, the bottleneck for BMP-2 production in E. coli is...

Descripción completa

Detalles Bibliográficos
Autores principales:	Manissorn, Juthatip, Tonsomboon, Khaow, Wangkanont, Kittikhun, Thongnuek, Peerapat
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9850730/ https://www.ncbi.nlm.nih.gov/pubmed/36687022 http://dx.doi.org/10.1021/acsomega.2c05802

Ejemplares similares

Collagen with simvastatin promotes cell metabolism in osteoblast-like SaOS-2 cells
por: Suthanthiran, Thanga Kumaran, et al.
Publicado: (2012)

Effects of silica–gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells
por: He, Wei, et al.
Publicado: (2018)

The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study
por: Brunello, Giulia, et al.
Publicado: (2021)

In Vitro Effects of PDGF Isoforms (AA, BB, AB and CC) on Migration and Proliferation of SaOS-2 Osteoblasts and on Migration of Human Osteoblasts
por: Colciago, Alessandra, et al.
Publicado: (2009)

G-protein Stimulatory α Subunit Is Involved in Osteogenic Activity in Osteoblastic Cell Line SaOS-2 Cells
por: Yamazaki, Miwa, et al.
Publicado: (2006)

Water Soluble Components of ‘Osteocare’ Promote Cell Proliferation, Differentiation, and Matrix Mineralization in Human Osteoblast-Like SaOS-2 Cells
por: Varma, Sandeep R., et al.
Publicado: (2014)

Effects of Processed Polygonum multiflorum with KIOM Patent on Bone Remodeling-Related Protein Expression in Human Osteoblast-Like SaOS-2 Cells
por: Kim, Dae Uk, et al.
Publicado: (2020)

Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells and Its Role in Proliferation
por: Cappadone, Concettina, et al.
Publicado: (2021)

Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study
por: Ayobian-Markazi, Nader, et al.
Publicado: (2012)

Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline
por: Boichuk, Sergei, et al.
Publicado: (2023)

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies
por: Esmaili, Iman, et al.
Publicado: (2018)

Attenuation Aβ(1-42)-induced neurotoxicity in neuronal cell by 660nm and 810nm LED light irradiation
por: Thammasart, Siriluk, et al.
Publicado: (2023)

Effect of Bioglass on Growth and Biomineralization of SaOS-2 Cells in Hydrogel after 3D Cell Bioprinting
por: Wang, Xiaohong, et al.
Publicado: (2014)

Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells
por: Khan, Mohammad Idreesh, et al.
Publicado: (2022)

Uptake of polyphosphate microparticles in vitro (SaOS-2 and HUVEC cells) followed by an increase of the intracellular ATP pool size
por: Müller, Werner E. G., et al.
Publicado: (2017)

Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane
por: Watchararot, Tanapong, et al.
Publicado: (2021)

Flouride Promotes Viability and Differentiation of Osteoblast-Like Saos-2 Cells Via BMP/Smads Signaling Pathway
por: Huo, Liangliang, et al.
Publicado: (2013)

Inactivation of the WNT5A Alternative Promoter B Is Associated with DNA Methylation and Histone Modification in Osteosarcoma Cell Lines U2OS and SaOS-2
por: Vaidya, Himani, et al.
Publicado: (2016)

Chicken chorioallantoic membrane as a reliable model to evaluate osteosarcoma—an experimental approach using SaOS2 cell line
por: Manjunathan, Reji, et al.
Publicado: (2015)

Blueberry Juice Antioxidants Protect Osteogenic Activity against Oxidative Stress and Improve Long-Term Activation of the Mineralization Process in Human Osteoblast-Like SaOS-2 Cells: Involvement of SIRT1
por: Domazetovic, Vladana, et al.
Publicado: (2020)

Thyroid-Stimulating Hormone Favors Runx2-Mediated Matrix Mineralization in HOS and SaOS2 Cells: An In Vitro and In Silico Approach
por: Govindan, Ramajayam, et al.
Publicado: (2022)

Alpha-tubulin enhanced renal tubular cell proliferation and tissue repair but reduced cell death and cell-crystal adhesion
por: Manissorn, Juthatip, et al.
Publicado: (2016)

Large-scale in vitro production, refolding and dimerization of PsbS in different microenvironments
por: Krishnan, Maithili, et al.
Publicado: (2017)

The hEag1 K(+) Channel Inhibitor Astemizole Stimulates Ca(2+) Deposition in SaOS-2 and MG-63 Osteosarcoma Cultures
por: Mészáros, Beáta, et al.
Publicado: (2022)

Hydrogelation of Regenerated Silk Fibroin via Gamma Irradiation
por: Thongnuek, Peerapat, et al.
Publicado: (2023)

Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells
por: Manissorn, Juthatip, et al.
Publicado: (2017)

RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth, invasion and migration of SaOS2 human osteosarcoma cells: a case control study
por: Zhao, Qinghua, et al.
Publicado: (2011)

Streptococcus agalactiae amylomaltase offers insight into the transglycosylation mechanism and the molecular basis of thermostability among amylomaltases
por: Tumhom, Suthipapun, et al.
Publicado: (2021)

The REFOLD database: a tool for the optimization of protein expression and refolding
por: Chow, Michelle K. M., et al.
Publicado: (2006)

SaO(2)/FiO(2 )ratio as risk stratification for patients with sepsis
por: Santana, Adriell Ramalho, et al.
Publicado: (2013)

OS-9 Buffer manager User's guide
por: Mato, P
Publicado: (1988)

OS-9 Buffer manager User's guide v2
por: Mato, P
Publicado: (1988)

Interaction of 8-anilinonaphthalene-1-sulfonate with SARS-CoV-2 main protease and its application as a fluorescent probe for inhibitor identification
por: Deetanya, Peerapon, et al.
Publicado: (2021)

Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1
por: Deetanya, Peerapon, et al.
Publicado: (2022)

Crosslinked Silk Fibroin/Gelatin/Hyaluronan Blends as Scaffolds for Cell-Based Tissue Engineering
por: Duangpakdee, Anongnart, et al.
Publicado: (2021)

Chloride promotes refolding of active Vibrio alkaline phosphatase through an inactive dimeric intermediate with an altered interface
por: Hjörleifsson, Jens Guðmundur, et al.
Publicado: (2018)

Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells
por: Reigada, Inés, et al.
Publicado: (2020)

Computational design of novel nanobodies targeting the receptor binding domain of variants of concern of SARS-CoV-2
por: Longsompurana, Phoomintara, et al.
Publicado: (2023)

Insights into the osteoblast precursor differentiation towards mature osteoblasts induced by continuous BMP-2 signaling
por: Zouani, Omar F., et al.
Publicado: (2013)

Discovery of C-12 dithiocarbamate andrographolide analogues as inhibitors of SARS-CoV-2 main protease: In vitro and in silico studies
por: Nutho, Bodee, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_7ooppavq7igcg51ksg2m9lntv7