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Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources

Nuclear transport is an essential part of eukaryotic cell function. Here, we present scFRAP, a model-assisted fluorescent recovery after photobleaching (FRAP)- based method to determine nuclear import and export rates independently in individual live cells. To overcome the inherent noise of single-c...

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Detalles Bibliográficos
Autores principales: Durrieu, Lucía, Bush, Alan, Grande, Alicia, Johansson, Rikard, Janzén, David, Katz, Andrea, Cedersund, Gunnar, Colman-Lerner, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852351/
https://www.ncbi.nlm.nih.gov/pubmed/36686393
http://dx.doi.org/10.1016/j.isci.2022.105906