Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies

Laminin polymerization is the major step in basement membranes assembly. Its failures cause laminin N-terminal domain lamininopathies including Pierson syndrome. We have employed cryo-electron microscopy to determine a 3.7 Å structure of the trimeric laminin polymer node containing α1, β1 and γ1 sub...

Descripción completa

Detalles Bibliográficos
Autores principales: Kulczyk, Arkadiusz W., McKee, Karen K., Zhang, Ximo, Bizukojc, Iwona, Yu, Ying Q., Yurchenco, Peter D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852560/
https://www.ncbi.nlm.nih.gov/pubmed/36658135
http://dx.doi.org/10.1038/s41467-023-36077-z
_version_ 1784872670998822912
author Kulczyk, Arkadiusz W.
McKee, Karen K.
Zhang, Ximo
Bizukojc, Iwona
Yu, Ying Q.
Yurchenco, Peter D.
author_facet Kulczyk, Arkadiusz W.
McKee, Karen K.
Zhang, Ximo
Bizukojc, Iwona
Yu, Ying Q.
Yurchenco, Peter D.
author_sort Kulczyk, Arkadiusz W.
collection PubMed
description Laminin polymerization is the major step in basement membranes assembly. Its failures cause laminin N-terminal domain lamininopathies including Pierson syndrome. We have employed cryo-electron microscopy to determine a 3.7 Å structure of the trimeric laminin polymer node containing α1, β1 and γ1 subunits. The structure reveals the molecular basis of calcium-dependent formation of laminin lattice, and provides insights into polymerization defects manifesting in human disease.
format Online
Article
Text
id pubmed-9852560
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-98525602023-01-21 Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies Kulczyk, Arkadiusz W. McKee, Karen K. Zhang, Ximo Bizukojc, Iwona Yu, Ying Q. Yurchenco, Peter D. Nat Commun Article Laminin polymerization is the major step in basement membranes assembly. Its failures cause laminin N-terminal domain lamininopathies including Pierson syndrome. We have employed cryo-electron microscopy to determine a 3.7 Å structure of the trimeric laminin polymer node containing α1, β1 and γ1 subunits. The structure reveals the molecular basis of calcium-dependent formation of laminin lattice, and provides insights into polymerization defects manifesting in human disease. Nature Publishing Group UK 2023-01-19 /pmc/articles/PMC9852560/ /pubmed/36658135 http://dx.doi.org/10.1038/s41467-023-36077-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kulczyk, Arkadiusz W.
McKee, Karen K.
Zhang, Ximo
Bizukojc, Iwona
Yu, Ying Q.
Yurchenco, Peter D.
Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title_full Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title_fullStr Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title_full_unstemmed Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title_short Cryo-EM reveals the molecular basis of laminin polymerization and LN-lamininopathies
title_sort cryo-em reveals the molecular basis of laminin polymerization and ln-lamininopathies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852560/
https://www.ncbi.nlm.nih.gov/pubmed/36658135
http://dx.doi.org/10.1038/s41467-023-36077-z
work_keys_str_mv AT kulczykarkadiuszw cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies
AT mckeekarenk cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies
AT zhangximo cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies
AT bizukojciwona cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies
AT yuyingq cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies
AT yurchencopeterd cryoemrevealsthemolecularbasisoflamininpolymerizationandlnlamininopathies

Ejemplares similares