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Purification of immune-active macrophage super enhancers by chemical cross-linked chromatin immune precipitation

Isolation of extraordinarily long-length super-enhancers (SEs) using typical chromatin immune precipitation (ChIP) techniques can lead to DNA breakage due to uncontrolled cross-linking. We present a redefined ChIP technique for SE purification. After controlled paraformaldehyde-based cross-linking,...

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Detalles Bibliográficos
Autores principales: Das, Sonali, Mukherjee, Sohitri, Ali, Nahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852664/
https://www.ncbi.nlm.nih.gov/pubmed/36638018
http://dx.doi.org/10.1016/j.xpro.2022.102004