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Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage

Alzheimer’s disease (AD) is a progressive neurodegenerative brain disorder and the most common cause of dementia in the elderly. The presence of large numbers of senile plaques, neurofibrillary tangles, and cerebral atrophy is the characteristic feature of AD. Amyloid β peptide (Aβ), derived from th...

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Autores principales: Kamikubo, Yuji, Jin, Hao, Zhou, Yiyao, Niisato, Kazue, Hashimoto, Yoshie, Takasugi, Nobumasa, Sakurai, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852844/
https://www.ncbi.nlm.nih.gov/pubmed/36683852
http://dx.doi.org/10.3389/fnmol.2022.1068990
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author Kamikubo, Yuji
Jin, Hao
Zhou, Yiyao
Niisato, Kazue
Hashimoto, Yoshie
Takasugi, Nobumasa
Sakurai, Takashi
author_facet Kamikubo, Yuji
Jin, Hao
Zhou, Yiyao
Niisato, Kazue
Hashimoto, Yoshie
Takasugi, Nobumasa
Sakurai, Takashi
author_sort Kamikubo, Yuji
collection PubMed
description Alzheimer’s disease (AD) is a progressive neurodegenerative brain disorder and the most common cause of dementia in the elderly. The presence of large numbers of senile plaques, neurofibrillary tangles, and cerebral atrophy is the characteristic feature of AD. Amyloid β peptide (Aβ), derived from the amyloid precursor protein (APP), is the main component of senile plaques. AD has been extensively studied using methods involving cell lines, primary cultures of neural cells, and animal models; however, discrepancies have been observed between these methods. Dissociated cultures lose the brain’s tissue architecture, including neural circuits, glial cells, and extracellular matrix. Experiments with animal models are lengthy and require laborious monitoring of multiple parameters. Therefore, it is necessary to combine these experimental models to understand the pathology of AD. An experimental platform amenable to continuous observation and experimental manipulation is required to analyze long-term neuronal development, plasticity, and progressive neurodegenerative diseases. In the current study, we provide a practical method to slice and cultivate rodent hippocampus to investigate the cleavage of APP and secretion of Aβ in an ex vivo model. Furthermore, we provide basic information on Aβ secretion using slice cultures. Using our optimized method, dozens to hundreds of long-term stable slice cultures can be coordinated simultaneously. Our findings are valuable for analyses of AD mouse models and senile plaque formation culture models.
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spelling pubmed-98528442023-01-21 Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage Kamikubo, Yuji Jin, Hao Zhou, Yiyao Niisato, Kazue Hashimoto, Yoshie Takasugi, Nobumasa Sakurai, Takashi Front Mol Neurosci Molecular Neuroscience Alzheimer’s disease (AD) is a progressive neurodegenerative brain disorder and the most common cause of dementia in the elderly. The presence of large numbers of senile plaques, neurofibrillary tangles, and cerebral atrophy is the characteristic feature of AD. Amyloid β peptide (Aβ), derived from the amyloid precursor protein (APP), is the main component of senile plaques. AD has been extensively studied using methods involving cell lines, primary cultures of neural cells, and animal models; however, discrepancies have been observed between these methods. Dissociated cultures lose the brain’s tissue architecture, including neural circuits, glial cells, and extracellular matrix. Experiments with animal models are lengthy and require laborious monitoring of multiple parameters. Therefore, it is necessary to combine these experimental models to understand the pathology of AD. An experimental platform amenable to continuous observation and experimental manipulation is required to analyze long-term neuronal development, plasticity, and progressive neurodegenerative diseases. In the current study, we provide a practical method to slice and cultivate rodent hippocampus to investigate the cleavage of APP and secretion of Aβ in an ex vivo model. Furthermore, we provide basic information on Aβ secretion using slice cultures. Using our optimized method, dozens to hundreds of long-term stable slice cultures can be coordinated simultaneously. Our findings are valuable for analyses of AD mouse models and senile plaque formation culture models. Frontiers Media S.A. 2023-01-06 /pmc/articles/PMC9852844/ /pubmed/36683852 http://dx.doi.org/10.3389/fnmol.2022.1068990 Text en Copyright © 2023 Kamikubo, Jin, Zhou, Niisato, Hashimoto, Takasugi and Sakurai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Kamikubo, Yuji
Jin, Hao
Zhou, Yiyao
Niisato, Kazue
Hashimoto, Yoshie
Takasugi, Nobumasa
Sakurai, Takashi
Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title_full Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title_fullStr Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title_full_unstemmed Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title_short Ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
title_sort ex vivo analysis platforms for monitoring amyloid precursor protein cleavage
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852844/
https://www.ncbi.nlm.nih.gov/pubmed/36683852
http://dx.doi.org/10.3389/fnmol.2022.1068990
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