Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach

Here, we describe a protocol for artificially generating hetero-oligomeric protein complexes from the homo-oligomers using a sequential denaturation-renaturation strategy, followed by a modified affinity chromatography protocol used for their purification. This protocol enables one to obtain a homog...

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Detalles Bibliográficos
Autores principales: Parui, Aasna L., Bose, Kakoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852955/
https://www.ncbi.nlm.nih.gov/pubmed/36853657
http://dx.doi.org/10.1016/j.xpro.2022.102036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852955/
https://www.ncbi.nlm.nih.gov/pubmed/36853657
http://dx.doi.org/10.1016/j.xpro.2022.102036

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