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Purification of hetero-oligomeric protein variants using a modified tandem affinity purification approach
Here, we describe a protocol for artificially generating hetero-oligomeric protein complexes from the homo-oligomers using a sequential denaturation-renaturation strategy, followed by a modified affinity chromatography protocol used for their purification. This protocol enables one to obtain a homog...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9852955/ https://www.ncbi.nlm.nih.gov/pubmed/36853657 http://dx.doi.org/10.1016/j.xpro.2022.102036 |