Immobilization of Firefly Bioluminescent System: Development and Application of Reagents

The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together. The addition of stabilizers dithiothreitol (DTT) and bovine serum albumin (BSA) to the reagent is a factor in achievi...

Descripción completa

Detalles Bibliográficos
Autores principales: Esimbekova, Elena N., Kirillova, Maria A., Kratasyuk, Valentina A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855680/
https://www.ncbi.nlm.nih.gov/pubmed/36671882
http://dx.doi.org/10.3390/bios13010047

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855680/
https://www.ncbi.nlm.nih.gov/pubmed/36671882
http://dx.doi.org/10.3390/bios13010047

Ejemplares similares