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Immobilization of Firefly Bioluminescent System: Development and Application of Reagents

The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together. The addition of stabilizers dithiothreitol (DTT) and bovine serum albumin (BSA) to the reagent is a factor in achievi...

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Detalles Bibliográficos
Autores principales:	Esimbekova, Elena N., Kirillova, Maria A., Kratasyuk, Valentina A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855680/ https://www.ncbi.nlm.nih.gov/pubmed/36671882 http://dx.doi.org/10.3390/bios13010047

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