Improved Split TEV GPCR β-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and β-arrestin Binding Motif Variants

G protein-coupled receptors (GPCRs) are major disease-relevant drug targets; robust monitoring of their activities upon drug treatment is key to drug discovery. The split TEV cell-based assay technique monitors the interaction of an activated GPCR with β-arrestin-2 through TEV protein fragment compl...

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Detalles Bibliográficos
Autores principales: Wu, Yuxin, von Hauff, Isabelle V., Jensen, Niels, Rossner, Moritz J., Wehr, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855867/
https://www.ncbi.nlm.nih.gov/pubmed/36671883
http://dx.doi.org/10.3390/bios13010048

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9855867/
https://www.ncbi.nlm.nih.gov/pubmed/36671883
http://dx.doi.org/10.3390/bios13010048

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