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Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness
Background: Mesenchymal stem cells (MSCs) have been used for ex vivo expansion of umbilical cord blood (UCB) hematopoietic stem cells (HSCs) to maintain their primitive characters and long-term reconstitution abilities during transplantation. Therapeutic effects of MSCs mainly rely on paracrine mech...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857343/ https://www.ncbi.nlm.nih.gov/pubmed/36672185 http://dx.doi.org/10.3390/cells12020250 |
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author | Teleb, Rasha S. Abdul-Hafez, Amal Othman, Amira Ahmed, Ahmed El-Abd Elsaid, Abdelrahman A. Arif, Hattan Zarea, Ahmed A. Abdulmageed, Mohammed Mohamed, Hend Ibrahim, Sherif Abdelfattah Thiruvenkataramani, Ranga P. Mohamed, Tarek Kanada, Masamitsu Madhukar, Burra V. Arellano, Myrna Gonzalez Sayed, Mohammed M. Qubaisy, Heba M. Omar, Said A. |
author_facet | Teleb, Rasha S. Abdul-Hafez, Amal Othman, Amira Ahmed, Ahmed El-Abd Elsaid, Abdelrahman A. Arif, Hattan Zarea, Ahmed A. Abdulmageed, Mohammed Mohamed, Hend Ibrahim, Sherif Abdelfattah Thiruvenkataramani, Ranga P. Mohamed, Tarek Kanada, Masamitsu Madhukar, Burra V. Arellano, Myrna Gonzalez Sayed, Mohammed M. Qubaisy, Heba M. Omar, Said A. |
author_sort | Teleb, Rasha S. |
collection | PubMed |
description | Background: Mesenchymal stem cells (MSCs) have been used for ex vivo expansion of umbilical cord blood (UCB) hematopoietic stem cells (HSCs) to maintain their primitive characters and long-term reconstitution abilities during transplantation. Therapeutic effects of MSCs mainly rely on paracrine mechanisms, including secretion of exosomes (Exos). The objective of this study was to examine the effect of cord blood plasma (CBP)-derived Exos (CBP Exos) and Placental MSCs-derived Exos (MSCs Exos) on the expansion of UCB HSCs to increase their numbers and keep their primitive characteristics. Methods: CD34(+) cells were isolated from UCB, cultured for 10 days, and the expanded HSCs were sub-cultured in semisolid methylcellulose media for primitive colony forming units (CFUs) assay. MSCs were cultured from placental chorionic plates. Results: CBP Exos and MSCs Exos compared with the control group significantly increased the number of total nucleated cells (TNCs), invitro expansion of CD34(+) cells, primitive subpopulations of CD34(+)38(+) and CD34(+)38(−)Lin(−) cells (p < 0.001). The expanded cells showed a significantly higher number of total CFUs in the Exos groups (p < 0.01). Conclusion: CBP- and placental-derived exosomes are associated with significant ex vivo expansion of UCB HSCs, while maintaining their primitive characters and may eliminate the need for transplantation of an additional unit of UCB. |
format | Online Article Text |
id | pubmed-9857343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98573432023-01-21 Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness Teleb, Rasha S. Abdul-Hafez, Amal Othman, Amira Ahmed, Ahmed El-Abd Elsaid, Abdelrahman A. Arif, Hattan Zarea, Ahmed A. Abdulmageed, Mohammed Mohamed, Hend Ibrahim, Sherif Abdelfattah Thiruvenkataramani, Ranga P. Mohamed, Tarek Kanada, Masamitsu Madhukar, Burra V. Arellano, Myrna Gonzalez Sayed, Mohammed M. Qubaisy, Heba M. Omar, Said A. Cells Article Background: Mesenchymal stem cells (MSCs) have been used for ex vivo expansion of umbilical cord blood (UCB) hematopoietic stem cells (HSCs) to maintain their primitive characters and long-term reconstitution abilities during transplantation. Therapeutic effects of MSCs mainly rely on paracrine mechanisms, including secretion of exosomes (Exos). The objective of this study was to examine the effect of cord blood plasma (CBP)-derived Exos (CBP Exos) and Placental MSCs-derived Exos (MSCs Exos) on the expansion of UCB HSCs to increase their numbers and keep their primitive characteristics. Methods: CD34(+) cells were isolated from UCB, cultured for 10 days, and the expanded HSCs were sub-cultured in semisolid methylcellulose media for primitive colony forming units (CFUs) assay. MSCs were cultured from placental chorionic plates. Results: CBP Exos and MSCs Exos compared with the control group significantly increased the number of total nucleated cells (TNCs), invitro expansion of CD34(+) cells, primitive subpopulations of CD34(+)38(+) and CD34(+)38(−)Lin(−) cells (p < 0.001). The expanded cells showed a significantly higher number of total CFUs in the Exos groups (p < 0.01). Conclusion: CBP- and placental-derived exosomes are associated with significant ex vivo expansion of UCB HSCs, while maintaining their primitive characters and may eliminate the need for transplantation of an additional unit of UCB. MDPI 2023-01-07 /pmc/articles/PMC9857343/ /pubmed/36672185 http://dx.doi.org/10.3390/cells12020250 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Teleb, Rasha S. Abdul-Hafez, Amal Othman, Amira Ahmed, Ahmed El-Abd Elsaid, Abdelrahman A. Arif, Hattan Zarea, Ahmed A. Abdulmageed, Mohammed Mohamed, Hend Ibrahim, Sherif Abdelfattah Thiruvenkataramani, Ranga P. Mohamed, Tarek Kanada, Masamitsu Madhukar, Burra V. Arellano, Myrna Gonzalez Sayed, Mohammed M. Qubaisy, Heba M. Omar, Said A. Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title | Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title_full | Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title_fullStr | Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title_full_unstemmed | Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title_short | Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness |
title_sort | cord blood plasma and placental mesenchymal stem cells-derived exosomes increase ex vivo expansion of human cord blood hematopoietic stem cells while maintaining their stemness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857343/ https://www.ncbi.nlm.nih.gov/pubmed/36672185 http://dx.doi.org/10.3390/cells12020250 |
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