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Semi-Site-Specific Primer PCR: A Simple but Reliable Genome-Walking Tool

Genome-walking has been frequently applied to molecular biology and related areas. Herein, a simple but reliable genome-walking technique, termed semi-site-specific primer PCR (3SP-PCR), is presented. The key to 3SP-PCR is the use of a semi-site-specific primer in secondary PCR that partially overla...

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Detalles Bibliográficos
Autores principales: Wei, Cheng, Lin, Zhiyu, Pei, Jinfeng, Pan, Hao, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9857434/
https://www.ncbi.nlm.nih.gov/pubmed/36661520
http://dx.doi.org/10.3390/cimb45010034