Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Targeting transgene integration into a safe genomic locus would be very important for gene therapy. We have generated lentivirus vectors containing the ribosomal RNA-recognising I-PpoI endonuclease fused to viral integrase, and transgene cassettes with target site homology arms to enhance insertion...

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Detalles Bibliográficos
Autores principales: Nousiainen, Alisa, Schenkwein, Diana, Ylä-Herttuala, Seppo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858087/
https://www.ncbi.nlm.nih.gov/pubmed/36662822
http://dx.doi.org/10.1371/journal.pone.0280894

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9858087/
https://www.ncbi.nlm.nih.gov/pubmed/36662822
http://dx.doi.org/10.1371/journal.pone.0280894

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