Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans

The auxin-inducible degron (AID) provides reversible, spatiotemporal control for the knockdown of target proteins. Here, we present a protocol for AID-mediated protein knockdown in Caenorhabditis elegans. We describe steps for generating the knock-in mutants using two CRISPR-Cas9 genome editing tech...

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Detalles Bibliográficos
Autores principales: Kurashina, Mizuki, Mizumoto, Kota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860162/
https://www.ncbi.nlm.nih.gov/pubmed/36640369
http://dx.doi.org/10.1016/j.xpro.2022.102028

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860162/
https://www.ncbi.nlm.nih.gov/pubmed/36640369
http://dx.doi.org/10.1016/j.xpro.2022.102028

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