Tandem mass tag-based thermal proteome profiling for the discovery of drug-protein interactions in cancer cells

Identification of effector targets is imperative to the characterization of the mechanisms of action of novel small molecules. Here, we describe steps to identify effector drug-protein interactions in lysates derived from cancer cell lines using a thermal proteome profiling (TPP) protocol. Building...

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Detalles Bibliográficos
Autores principales: Johnson, Fraser D., Hughes, Christopher S., Liu, Alvin, Lockwood, William W., Morin, Gregg B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860163/
https://www.ncbi.nlm.nih.gov/pubmed/36856765
http://dx.doi.org/10.1016/j.xpro.2022.102012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9860163/
https://www.ncbi.nlm.nih.gov/pubmed/36856765
http://dx.doi.org/10.1016/j.xpro.2022.102012

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