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Detection of Tobacco Bacterial Wilt Caused by Ralstonia solanacearum by Combining Polymerase Chain Reaction with an α-Hemolysin Nanopore

Tobacco bacterial wilt is a serious disease caused by the soil-borne bacterium Ralstonia solanacearum (R. solanacearum). Herein, a rapid and purification-free α-hemolysin (α-HL) nanopore-sensing strategy based on polymerase chain reaction (PCR) and lambda exonuclease digestion was established to det...

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Detalles Bibliográficos
Autores principales: Wang, Ying, Li, Yusen, Zhou, Xin, Zhang, Wenna, Zhang, Shusheng, Xi, Dongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9863824/
https://www.ncbi.nlm.nih.gov/pubmed/36678085
http://dx.doi.org/10.3390/nano13020332