Protocol for semisynthesis of serotonylated histone H3 by rapid protein desulfurization in tandem with native chemical ligation

Here, we present a protocol of rapid protein desulfurization in tandem with native chemical ligation for facile syntheses of proteins with site-specific modifications. We describe using sodium tetraethylborate (NaBEt(4)) to carry out this desulfurization in an add-and-done manner under ambient condi...

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Detalles Bibliográficos
Autores principales: Sun, Zhenquan, Wei, Tongyao, Cao, Yihui, Li, Xuechen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867976/
https://www.ncbi.nlm.nih.gov/pubmed/36825812
http://dx.doi.org/10.1016/j.xpro.2022.102042

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9867976/
https://www.ncbi.nlm.nih.gov/pubmed/36825812
http://dx.doi.org/10.1016/j.xpro.2022.102042

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