Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children

OBJECTIVES: To develop a rapid and low-cost method for 16S rDNA nanopore sequencing. METHODS: This was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Yinghu, Mao, Lingfeng, Lai, Dengming, Xu, Weize, Zhang, Yuebai, Wu, Sihao, Yang, Di, Zhao, Shaobo, Liu, Zhicong, Xiao, Yi, Tang, Yi, Meng, Xiaofang, Wang, Min, Shi, Jueliang, Chen, Qixing, Shu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868273/
https://www.ncbi.nlm.nih.gov/pubmed/36699719
http://dx.doi.org/10.3389/fcimb.2022.1001607

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868273/
https://www.ncbi.nlm.nih.gov/pubmed/36699719
http://dx.doi.org/10.3389/fcimb.2022.1001607

Ejemplares similares