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Improved targeting of the 16S rDNA nanopore sequencing method enables rapid pathogen identification in bacterial pneumonia in children

OBJECTIVES: To develop a rapid and low-cost method for 16S rDNA nanopore sequencing. METHODS: This was a prospective study on a 16S rDNA nanopore sequencing method. We developed this nanopore barcoding 16S sequencing method by adding barcodes to the 16S primer to reduce the reagent cost and simplify...

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Detalles Bibliográficos
Autores principales:	Chen, Yinghu, Mao, Lingfeng, Lai, Dengming, Xu, Weize, Zhang, Yuebai, Wu, Sihao, Yang, Di, Zhao, Shaobo, Liu, Zhicong, Xiao, Yi, Tang, Yi, Meng, Xiaofang, Wang, Min, Shi, Jueliang, Chen, Qixing, Shu, Qiang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Cellular and Infection Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868273/ https://www.ncbi.nlm.nih.gov/pubmed/36699719 http://dx.doi.org/10.3389/fcimb.2022.1001607

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