Cargando…

SIK3 and Wnk converge on Fray to regulate glial K(+) buffering and seizure susceptibility

Glial cells play a critical role in maintaining homeostatic ion concentration gradients. Salt-inducible kinase 3 (SIK3) regulates a gene expression program that controls K(+) buffering in glia, and upregulation of this pathway suppresses seizure behavior in the eag, Shaker hyperexcitability mutant....

Descripción completa

Detalles Bibliográficos
Autores principales: Lones, Lorenzo, DiAntonio, Aaron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9870106/
https://www.ncbi.nlm.nih.gov/pubmed/36626385
http://dx.doi.org/10.1371/journal.pgen.1010581
Descripción
Sumario:Glial cells play a critical role in maintaining homeostatic ion concentration gradients. Salt-inducible kinase 3 (SIK3) regulates a gene expression program that controls K(+) buffering in glia, and upregulation of this pathway suppresses seizure behavior in the eag, Shaker hyperexcitability mutant. Here we show that boosting the glial SIK3 K(+) buffering pathway suppresses seizures in three additional molecularly diverse hyperexcitable mutants, highlighting the therapeutic potential of upregulating glial K(+) buffering. We then explore additional mechanisms regulating glial K(+) buffering. Fray, a transcriptional target of the SIK3 K(+) buffering program, is a kinase that promotes K(+) uptake by activating the Na(+)/K(+)/Cl(-) co-transporter, Ncc69. We show that the Wnk kinase phosphorylates Fray in Drosophila glia and that this activity is required to promote K(+) buffering. This identifies Fray as a convergence point between the SIK3-dependent transcriptional program and Wnk-dependent post-translational regulation. Bypassing both regulatory mechanisms via overexpression of a constitutively active Fray in glia is sufficient to robustly suppress seizure behavior in multiple Drosophila models of hyperexcitability. Finally, we identify cortex glia as a critical cell type for regulation of seizure susceptibility, as boosting K(+) buffering via expression of activated Fray exclusively in these cells is sufficient to suppress seizure behavior. These findings highlight Fray as a key convergence point for distinct K(+) buffering regulatory mechanisms and cortex glia as an important locus for control of neuronal excitability.