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Protocol to study presynaptic protein synthesis in ex vivo mouse hippocampal slices using HaloTag technology
Presynaptic boutons in the mammalian brain are typically small and difficult to manipulate and study. Here, we present a protocol applying HaloTag self-labeling technology to detect de novo local protein synthesis in intact presynaptic mossy fiber boutons from acute mouse hippocampal slices. We desc...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871327/ https://www.ncbi.nlm.nih.gov/pubmed/36853723 http://dx.doi.org/10.1016/j.xpro.2022.101986 |