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Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45(+) tumor-infiltrating lymphocyte...

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Detalles Bibliográficos
Autores principales: Kalfeist, Laura, Petit, Stacy, Galland, Loïck, Ghiringhelli, François, Ladoire, Sylvain, Limagne, Emeric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871328/
https://www.ncbi.nlm.nih.gov/pubmed/36856770
http://dx.doi.org/10.1016/j.xpro.2022.101951