Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45(+) tumor-infiltrating lymphocyte...

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Autores principales: Kalfeist, Laura, Petit, Stacy, Galland, Loïck, Ghiringhelli, François, Ladoire, Sylvain, Limagne, Emeric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871328/
https://www.ncbi.nlm.nih.gov/pubmed/36856770
http://dx.doi.org/10.1016/j.xpro.2022.101951
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author Kalfeist, Laura
Petit, Stacy
Galland, Loïck
Ghiringhelli, François
Ladoire, Sylvain
Limagne, Emeric
author_facet Kalfeist, Laura
Petit, Stacy
Galland, Loïck
Ghiringhelli, François
Ladoire, Sylvain
Limagne, Emeric
author_sort Kalfeist, Laura
collection PubMed
description The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45(+) tumor-infiltrating lymphocytes, cancer-associated fibroblasts, and tumor cells. The protocol includes four steps: generation of 4T1 tumors, tumor collection and digestion, magnetic sorting of the different populations, and phenotypic validation of sorted cells. For complete details on the use and execution of this protocol, please refer to Limagne et al. (2022).(1)
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spelling pubmed-98713282023-01-25 Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS Kalfeist, Laura Petit, Stacy Galland, Loïck Ghiringhelli, François Ladoire, Sylvain Limagne, Emeric STAR Protoc Protocol The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45(+) tumor-infiltrating lymphocytes, cancer-associated fibroblasts, and tumor cells. The protocol includes four steps: generation of 4T1 tumors, tumor collection and digestion, magnetic sorting of the different populations, and phenotypic validation of sorted cells. For complete details on the use and execution of this protocol, please refer to Limagne et al. (2022).(1) Elsevier 2023-01-19 /pmc/articles/PMC9871328/ /pubmed/36856770 http://dx.doi.org/10.1016/j.xpro.2022.101951 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kalfeist, Laura
Petit, Stacy
Galland, Loïck
Ghiringhelli, François
Ladoire, Sylvain
Limagne, Emeric
Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title_full Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title_fullStr Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title_full_unstemmed Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title_short Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS
title_sort simultaneous isolation of cd45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by macs
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871328/
https://www.ncbi.nlm.nih.gov/pubmed/36856770
http://dx.doi.org/10.1016/j.xpro.2022.101951
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