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Protocol for identification and computational analysis of human natural killer cells using flow cytometry and R

Identifying differential protein expression is routinely used to delineate natural killer (NK) cells from various sample cohorts. This protocol describes key steps for NK cell analysis: identifying human NK cells using flow gating, data export from FlowJo, data loading in R, dimensionality reduction...

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Detalles Bibliográficos
Autores principales: Kroll, Kyle, Reeves, R. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9871333/
https://www.ncbi.nlm.nih.gov/pubmed/36853664
http://dx.doi.org/10.1016/j.xpro.2023.102044